Below you will find references to methodological papers written by our staff. These describe the methods and tools used by Delta Phenomics. PDF copies are available on request.

Phenotyping 

Tegelenbosch, R.A.J.; van Dam, E.A.; Noldus, L.P.J.J.; van der Harst,  J.E., ter Braak C.J.F.; Spruijt, B.M. (2011). Automated recognition of ten specific rat behaviors from overhead video. Society for Neuroscience (SfN) poster, Washington, DC, USA, 12-16 November 2011.

de Heer, R.C.; Schenke, M.; Mellace, M.; van Stipdonk, N.W.J.; Kamphuis, W.; Hol, E.M.; Spruijt, B.M. (2011) Early changes in spontaneour behaviour of mouse models for human neurodegenerative diseases. ENP Abstract, Lunteren, The Netherlands, 30 May - 1 June 2011.

Mellace, M.; de Heer, R.C.; van Stipdonk, N.W.J.; Smith, D.L.; Bates, G.P.; Spruijt, B.M. (2011) Early spontaneous behavioural signs of Huntington's disease in R6/2 mice detected in home cage situation. ENP Poster, Lunteren, The Netherlands, 30 May - 1 June 2011.

van der Harst, J.E.; de Heer, R.C.; Spruijt, B.M. (2011) Automated home cage ethology: biologically relevant and efficient, but complex. Abstract, 2nd ToK conference of CompCog: Current challenges and applications of comparative cognition, Prague, Czech Republic, 25-27 May 2011. 

de Heer, R.C.; Mellace, M.; van Stipdonk, N.W.J.; Spruijt, B.M. (2010) Age related changes in learning using an automated reversal learning test in a home cage environment. FENS Abstract, vol.5, 058.4 (Amsterdam, The Netherlands, 3-7 July 2010).

Casadei, N.; Nuber, S.; Bornemann, A.; Schell, H.; Diepenbroek, M.; Ciossek, T.; Mellace, M.; Krüger, R.; Jensen, P.H.; Kahle, P.; Riess, O. (2010). Characterization of Synphilin1/A30P-alpha-Synuclein Double Transgenic Mice as a Model of Parkinson’s Disease. 83 Annual Congress of the Deutsche Gesellschaft fur Neurologie, 21-25 September 2010, Mannheim, Germany. 

Mellace, M.; de Heer R.C.; Fekas, D.; van Stipdonk, N.W.J.; Spruijt, B.M. (2010) Early behavioural signs of a Parkinson's disease transgenic mouse model automatically detected in a home cage situation. FENS Abstract, vol.5, 197.30, (Amsterdam, The Netherlands, 3-7 July 2010).

Spruijt, B.M.; de Heer, R.C.; van der Harst, J.E. (2010) Spontaneous behavior in the home cage: a new test environment for measuring neuropathic pain. Abstract Proceedings of Measuring Behavior 2010 (Eindhoven, The Netherlands, 24-27 August, 2010) p.85-87

Dalm, S.; de Visser, L.; Spruijt, B.M.; Oitzl, M.S. (2009). Repeated rat exposure inhibits the circadian activity patterns of C57BL/6J mice in the home cage. Behavioural Brain Research 196, 84-92.

van Gassen, K.L.; Hessel, E.V.; Ramakers, G.M.; Notenboom, R.G.; Wolterink-Donselaar, I.G.; Brakkee, J.H.; Godschalk, T.C.; Qiao, X.; Spruijt, B.M.; van Nieuwenhuizen, O.; de Graan, P.N. (2009). Characterization of febrile seizures and febrile seizure susceptibility in mouse inbred strains. Genes Brain and Behavior 7, 578-586. 

de Heer, R.C.; Schenke, M.; Kuurman, W.W.; Spruijt, B.M. (2008). Learning (in) the PhenoTyper: an integrative approach to conducting cognitive behavioural challenges in a home cage environment. Proceedings of Measuring Behavior 2008 (Maastricht, The Netherlands, August 26-29, 2008), p.57.

Visser, L. de (2008). Home sweet home: Home cage testing for behavioural phenotyping of mice. Ph.D. Thesis, Utrecht University 186 pp.

Kas, M.J.; de Mooij-van Malsen, A.J.; Olivier, B.; Spruijt, B.M.; van Ree, J.M. (2008). Differential genetic regulation of motor activity and anxiety-related behaviors in mice using an automated home cage task. Behavioural Neuroscience 122, 769-76.

Visser, L. de; Bos van den, R.; Stoker, A.; Kas, M.; Spruijt, B. (2007). Effects of genetic background and environmental novelty on wheel running as a rewarding behaviour in mice. Behavioural Brain Research 177, 290-297.

Spruijt, B.; de Visser, L. (2006). Advanced behavioural screening: automated home cage ethology. Drug Discovery Today: Technologies 3, 231-237.

Visser, L. de; Bos van den, R.; Kuurman, W.W.; Kas, M.J.H.; Spruijt, B.M. (2006). Novel approach to the behavioural characteristics of inbred mice: automated home cage observations. Genes Brain and Behavior 5, 458-466.

Visser, L. de; Bos, R. van den; Kuurman, W.W.; Spruijt, B.M. (2005). Multi-level analysis of mouse behavior in a home cage environment using PhenoLab. Proceedings of Measuring Behavior 2005, 5th International Conference on Methods and Techniques in Behavioral Research (Wageningen, 30 August - 2 September 2005), 73-76.

Visser, L. de; Bos van den, R.; Spruijt, B.M. (2005). Automated home cage observations as a tool to measure the effects of wheel running on cage floor locomotion. Behavioural Brain Research 160, 382-388.

Video tracking, behavior recognition and analysis

Noldus, L.P.J.J.; Spink, A.J.; Tegelenbosch, R.A.J. (2001). EthoVision: a versatile video tracking system for automation of behavioral experiments. Behavior Research Methods, Instruments & Computers 33, 398-414.

Spink, A.J.; Tegelenbosch, R.A.J.; Buma, M.O.S.; Noldus, L.P.J.J. (2001). The EthoVision video tracking system: a tool for behavioral phenotyping of transgenic mice. Physiology & Behavior 73, 731-744.

Rousseau, J.B.I.; Lochem, P.B.A. van; Gispen, W.H.; Spruijt, B.M. (2000). Classification of rat behavior with an image-processing method and a neural network. Behavior Research Methods, Instruments & Computers 32, 63-71.

Spruijt, B.M.; Hol, T.; Rousseau, J. (1992). Approach, avoidance and contact behavior of individually recognized animals automatically quantified with an imaging technique. Physiology & Behavior 51, 747-752.

Spruijt, B.M. (1991) Progressive decline in social attention in aging rats: an informational-statistical method. Neurobiology of Aging 13, 145-151.

Spruijt, B.M.; Gispen, W.H. (1984). Behavioral sequences as an easily quantifiable parameter in experimental studies. Physiology & Behavior 32, 707-710.

Spruijt, B.M.; Gispen, W.H. (1983). Prolonged animal observation by use of digitized video displays. Pharmacology Biochemistry & Behavior 19, 765 769.

Social stress model

Kamal, A.; van der Harst, J.E.; Kapteijn, C.M.; Baars, A.J.M.; Spruijt, B.M.; Ramakers, G.M.J (2010). Announced reward counteracts the effects of chronic social stress on anticipatory behavior and hippocampal synaptic plasticity in rats. Experimental brain research 201, 641-651.

Cornelisse, L.N.; van der Harst, J.E.; Lodder, J.C.; Baarendse, P.J.J.; Timmerman, A.J.; Mansvelder, H.D.; Spruijt, B.M.; Brussaard, A.B. (2008). Reduced 5-HT1A- and GABAB receptor function in dorsal raphé upon chronic fluoxetine treatment correlates with relief from symptoms of depression in rats. Journal of Neurophysiology 98, 196-204.

Van der Harst, J.E.; Baars, J.M.; Spruijt, B.M. (2005). Announced rewards counteract the impairment of anticipatory behaviour in socially stressed rats. Behavioural Brain Research 161, 183-189.

Van der Harst, J.E.; Lubbers, M., Eijkhoudt, M.; Spruijt, B.M. (2005) Monitoring the effects of social defeat in mice by automated observation in the home-cage and observer-based scoring during a social-interest test. Measuring Behavior 2005, 5th International Conference on Methods and Techniques in Behavioral Research (Wageningen, 30 August - 2 September 2005, Invited speaker)

Von Frijtag, J.C.; van den Bos, R.; Spruijt, B.M. (2002). Imipramine restores the long-term impairment of appetitive behavior in socially stressed rats. Psychopharmacology 162, 232-238.

Von Frijtag J.C.; Reijmers, L.G.J.E.; Van der Harst, J.E.; Leus, I.E.; Van den Bos, R.; Spruijt, B.M. (2000). Defeat followed by individual housing results in long-term impaired reward- en cognition-related behaviours in rats. Behavioural Brain Research 117, 137-146.